Genotyping and primer details


Genotyping and primer details for 040D08 - At1g51960
Line ID 040D08
Locus At1g51960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19311316 of line 040D08 Chr1:19311316
   
Insertion allele amplicon 8474 - CW15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW15: 5'-ATTTTGGCAAATGGTTTTGTTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19310727
   
wt amplicon (over InsPos) 01dy - CW15
wt amplicon start Chr1:19310727
wt amplicon end Chr1:19311807
wt amplicon size 1081 bp
Locus specific primer CW15: 5'-ATTTTGGCAAATGGTTTTGTTAAG-3'
2nd locus specific primer 01dy: 5'-TAGAACTGCTTGATGTTTGCTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11