Genotyping and primer details


Genotyping and primer details for 045F05 - At1g52420
Line ID 045F05
Locus At1g52420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19530670 of line 045F05 Chr1:19530670
   
Insertion allele amplicon 8474 - 05in
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05in: 5'-AAGAATCTCAAAGGAATCACAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19530150
   
wt amplicon (over InsPos) 01ji - 05in
wt amplicon start Chr1:19530150
wt amplicon end Chr1:19531114
wt amplicon size 965 bp
Locus specific primer 05in: 5'-AAGAATCTCAAAGGAATCACAAGG-3'
2nd locus specific primer 01ji: 5'-CAAAGACGGTATCTCACAATTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11