Genotyping and primer details


Genotyping and primer details for 046E12 - At5g24380
Line ID 046E12
Locus At5g24380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8325014 of line 046E12 Chr5:8325014
   
Insertion allele amplicon 3144 - FL94
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FL94: 5'-TGTTGCTTGTTACAGCATCTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8324409
   
Insertion allele amplicon 8474 - MJ15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ15: 5'-CGAAGTTATAAAGCCCATCTCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8325432
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11