Genotyping and primer details


Genotyping and primer details for 050C04 - At1g26150
Line ID 050C04
Locus At1g26150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9042620 of line 050C04 Chr1:9042620
   
Insertion allele amplicon 8474 - FI12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FI12: 5'-GAGGTGATGGAGTAGGACGTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9042144
   
Insertion allele amplicon 3144 - RK60
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer RK60: 5'-GAAGCCTGTTGTCCGAGTATTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9043103
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11