Genotyping and primer details


Genotyping and primer details for 050D12 - At4g36910
Line ID 050D12
Locus At4g36910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17392061 of line 050D12 Chr4:17392061
   
Insertion allele amplicon 8474 - IN08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IN08: 5'-ATTATTGTGCATGGTAATTGCGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17391730
   
Insertion allele amplicon 3144 - IN09
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IN09: 5'-AGTTCATCAGATTCTTTGGAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17392514
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11