Genotyping and primer details


Genotyping and primer details for 051D01 - At2g29110
Line ID 051D01
Locus At2g29110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12507546 of line 051D01 Chr2:12507546
   
Insertion allele amplicon 8474 - AJ13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ13: 5'-AACATAGAGTCAACACCGACTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12508211
   
Insertion allele amplicon 3144 - AL09
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AL09: 5'-ATATCGAATCTTCGGAATCATCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12507158
   
wt amplicon (over InsPos) AL09 - AJ13
wt amplicon start Chr2:12507158
wt amplicon end Chr2:12508211
wt amplicon size 1054 bp
Locus specific primer AJ13: 5'-AACATAGAGTCAACACCGACTTCC-3'
2nd locus specific primer AL09: 5'-ATATCGAATCTTCGGAATCATCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11