Genotyping and primer details


Genotyping and primer details for 052E04 - At3g07540
Line ID 052E04
Locus At3g07540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2407208 of line 052E04 Chr3:2407208
   
Insertion allele amplicon 3144 - 04fm
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 04fm: 5'-TAGAGAACATCGGAGGTAGAAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2406935
   
wt amplicon (over InsPos) 08gf - 04fm
wt amplicon start Chr3:2406935
wt amplicon end Chr3:2407638
wt amplicon size 704 bp
Locus specific primer 04fm: 5'-TAGAGAACATCGGAGGTAGAAGGA-3'
2nd locus specific primer 08gf: 5'-AGATTCTTTTGAAAGAGAGGACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11