Genotyping and primer details


Genotyping and primer details for 053B12 - At3g02040
Line ID 053B12
Locus At3g02040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:349662 of line 053B12 Chr3:349662
   
Insertion allele amplicon 8474 - 02fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02fp: 5'-ATCAATCTCACAGGAAGGAAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:349975
   
wt amplicon (over InsPos) 14gf - 02fp
wt amplicon start Chr3:349249
wt amplicon end Chr3:349975
wt amplicon size 727 bp
Locus specific primer 02fp: 5'-ATCAATCTCACAGGAAGGAAAGAA-3'
2nd locus specific primer 14gf: 5'-GATCTTCCCTTCCTTTGACTTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11