Genotyping and primer details


Genotyping and primer details for 054C03 - At1g24807
Line ID 054C03
Locus At1g24807 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8772646 of line 054C03 Chr1:8772646
   
Insertion allele amplicon 8474 - 48oc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 48oc: 5'-TCGAGCTCATCACTAGGAAATGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8772025
   
Insertion allele amplicon 8474 - PC30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PC30: 5'-CATTTAAGCTGTGGATGCATAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8772313
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11