Genotyping and primer details


Genotyping and primer details for 055G07 - At3g13160
Line ID 055G07
Locus At3g13160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4231278 of line 055G07 Chr3:4231278
   
Insertion allele amplicon 8474 - KA17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KA17: 5'-CCTTGTTCTCAATCTCATCAATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4230560
   
wt amplicon (over InsPos) 01dn - KA17
wt amplicon start Chr3:4230560
wt amplicon end Chr3:4231598
wt amplicon size 1039 bp
Locus specific primer KA17: 5'-CCTTGTTCTCAATCTCATCAATCA-3'
2nd locus specific primer 01dn: 5'-ATTCCTTCTAAGATTCGTCGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11