Genotyping and primer details


Genotyping and primer details for 062G02 - At4g28556
Line ID 062G02
Locus At4g28556 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14115257 of line 062G02 Chr4:14115257
   
Insertion allele amplicon 8474 - YL02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer YL02: 5'-TGAGGTAGAATCTTCGTCTTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14115579
   
wt amplicon (over InsPos) 49vh - YL02
wt amplicon start Chr4:14114728
wt amplicon end Chr4:14115579
wt amplicon size 852 bp
Locus specific primer YL02: 5'-TGAGGTAGAATCTTCGTCTTTGGT-3'
2nd locus specific primer 49vh: 5'-TTGGTTTCCACAAAGAGAAGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11