Genotyping and primer details


Genotyping and primer details for 064G04 - At3g44340
Line ID 064G04
Locus At3g44340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16014439 of line 064G04 Chr3:16014439
   
Insertion allele amplicon 8474 - NA11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NA11: 5'-GCTCCATCTTGTAGTTTGTCATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16014064
   
wt amplicon (over InsPos) 31go - NA11
wt amplicon start Chr3:16014064
wt amplicon end Chr3:16015017
wt amplicon size 954 bp
Locus specific primer NA11: 5'-GCTCCATCTTGTAGTTTGTCATCA-3'
2nd locus specific primer 31go: 5'-AAGAACAACTGGAGGACAGGTAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11