Genotyping and primer details


Genotyping and primer details for 065E11 - At3g27610
Line ID 065E11
Locus At3g27610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10226674 of line 065E11 Chr3:10226674
   
Insertion allele amplicon 8474 - 03uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03uj: 5'-TGACTGATTCTGAGGTGCTTAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10227039
   
wt amplicon (over InsPos) 26vg - 03uj
wt amplicon start Chr3:10226194
wt amplicon end Chr3:10227039
wt amplicon size 846 bp
Locus specific primer 03uj: 5'-TGACTGATTCTGAGGTGCTTAATG-3'
2nd locus specific primer 26vg: 5'-TAACAATCCTTGCTGCAGTATCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11