Genotyping and primer details


Genotyping and primer details for 067F03 - At5g53890
Line ID 067F03
Locus At5g53890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21879894 of line 067F03 Chr5:21879894
   
Insertion allele amplicon 8474 - KE26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE26: 5'-CCATAAGTCTAGCCATTGGGATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21879285
   
Insertion allele amplicon 3144 - TN16
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer TN16: 5'-TTGTTGTTGAACAGACTCCATAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21880342
   
wt amplicon (over InsPos) TN16 - KE26
wt amplicon start Chr5:21879285
wt amplicon end Chr5:21880342
wt amplicon size 1058 bp
Locus specific primer KE26: 5'-CCATAAGTCTAGCCATTGGGATTA-3'
2nd locus specific primer TN16: 5'-TTGTTGTTGAACAGACTCCATAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11