Genotyping and primer details


Genotyping and primer details for 069B12 - At5g65160
Line ID 069B12
Locus At5g65160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26032052 of line 069B12 Chr5:26032052
   
Insertion allele amplicon 8474 - 04ke
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ke: 5'-GTTGTTGAGCCTCTGATAATCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26031489
   
wt amplicon (over InsPos) 07ke - 04ke
wt amplicon start Chr5:26031489
wt amplicon end Chr5:26032401
wt amplicon size 912 bp
Locus specific primer 04ke: 5'-GTTGTTGAGCCTCTGATAATCCTC-3'
2nd locus specific primer 07ke: 5'-TGATGATGCTTTGTCTAGATGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11