Genotyping and primer details


Genotyping and primer details for 069C05 - At5g09960
Line ID 069C05
Locus At5g09960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3107270 of line 069C05 Chr5:3107270
   
Insertion allele amplicon 3144 - 04kk
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 04kk: 5'-GAAAAAGAAAAATGGATTGGAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3107769
   
wt amplicon (over InsPos) 04lb - 04kk
wt amplicon start Chr5:3106653
wt amplicon end Chr5:3107769
wt amplicon size 1117 bp
Locus specific primer 04kk: 5'-GAAAAAGAAAAATGGATTGGAACA-3'
2nd locus specific primer 04lb: 5'-GCTAATCAAGCTCCAAGCACTACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11