Genotyping and primer details


Genotyping and primer details for 069E09 - At5g64840
Line ID 069E09
Locus At5g64840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25919518 of line 069E09 Chr5:25919518
   
Insertion allele amplicon 3144 - 05ax
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 05ax: 5'-TCTCTGCAACAAATCAAATTCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25919019
   
wt amplicon (over InsPos) 04aq - 05ax
wt amplicon start Chr5:25919019
wt amplicon end Chr5:25919824
wt amplicon size 806 bp
Locus specific primer 05ax: 5'-TCTCTGCAACAAATCAAATTCATC-3'
2nd locus specific primer 04aq: 5'-CGCTCAAAACGATAGAGAGAAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11