Genotyping and primer details


Genotyping and primer details for 070C11 - At2g47410
Line ID 070C11
Locus At2g47410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19450421 of line 070C11 Chr2:19450421
   
Insertion allele amplicon 8474 - 09uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09uv: 5'-ATGGAAAGCAATAGCTCACTTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19450865
   
wt amplicon (over InsPos) 45vn - 09uv
wt amplicon start Chr2:19450102
wt amplicon end Chr2:19450865
wt amplicon size 764 bp
Locus specific primer 09uv: 5'-ATGGAAAGCAATAGCTCACTTCAT-3'
2nd locus specific primer 45vn: 5'-TGGTATCATGACTTGTATTGGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11