Genotyping and primer details


Genotyping and primer details for 071B06 - At1g05700
Line ID 071B06
Locus At1g05700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1711402 of line 071B06 Chr1:1711402
   
Insertion allele amplicon 8474 - KE22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE22: 5'-ATCTTAGTCTCAAACCGAACCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1710880
   
Insertion allele amplicon 3144 - KE23
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KE23: 5'-TAATAACTCGGATGGAACTGAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1711787
   
wt amplicon (over InsPos) KE23 - KE22
wt amplicon start Chr1:1710880
wt amplicon end Chr1:1711787
wt amplicon size 908 bp
Locus specific primer KE22: 5'-ATCTTAGTCTCAAACCGAACCAGA-3'
2nd locus specific primer KE23: 5'-TAATAACTCGGATGGAACTGAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11