Genotyping and primer details


Genotyping and primer details for 072F02 - At4g02400
Line ID 072F02
Locus At4g02400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1057723 of line 072F02 Chr4:1057723
   
Insertion allele amplicon 8474 - ME27
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ME27: 5'-TGGTTTTCGGGTGAAAATAGTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1058268
   
Insertion allele amplicon 3144 - ME28
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer ME28: 5'-CTCAAGCAGAGCTTATACAACGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1057135
   
wt amplicon (over InsPos) 19km - ME27
wt amplicon start Chr4:1057299
wt amplicon end Chr4:1058268
wt amplicon size 970 bp
Locus specific primer ME27: 5'-TGGTTTTCGGGTGAAAATAGTTAG-3'
2nd locus specific primer 19km: 5'-CCTTCATGGATGGTTAGAGAACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11