Genotyping and primer details


Genotyping and primer details for 075D09 - At3g02930
Line ID 075D09
Locus At3g02930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:657256 of line 075D09 Chr3:657256
   
Insertion allele amplicon 8474 - 05aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05aq: 5'-TCAGAGTTAATACGGTTGAAGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:656592
   
Insertion allele amplicon 8474 - 13ax
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ax: 5'-TATTCATTTCTTTCCCCATTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:657579
   
wt amplicon (over InsPos) 13ax - 05aq
wt amplicon start Chr3:656592
wt amplicon end Chr3:657579
wt amplicon size 988 bp
Locus specific primer 05aq: 5'-TCAGAGTTAATACGGTTGAAGGCT-3'
2nd locus specific primer 13ax: 5'-TATTCATTTCTTTCCCCATTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11