Genotyping and primer details


Genotyping and primer details for 075E09 - At3g17850
Line ID 075E09
Locus At3g17850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6115576 of line 075E09 Chr3:6115576
   
Insertion allele amplicon 8474 - RK50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK50: 5'-TACTTCCTGGAAAAGACAGACGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6114740
   
wt amplicon (over InsPos) 01sq - RK50
wt amplicon start Chr3:6114740
wt amplicon end Chr3:6115984
wt amplicon size 1245 bp
Locus specific primer RK50: 5'-TACTTCCTGGAAAAGACAGACGAT-3'
2nd locus specific primer 01sq: 5'-GCTCACTCTATTGGAAAATCGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11