Genotyping and primer details


Genotyping and primer details for 075F01 - At1g53380
Line ID 075F01
Locus At1g53380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19913359 of line 075F01 Chr1:19913359
   
Insertion allele amplicon 8474 - 03wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03wu: 5'-GATGGTTCATAGACCAGCAATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19912868
   
wt amplicon (over InsPos) 16xd - 03wu
wt amplicon start Chr1:19912868
wt amplicon end Chr1:19914070
wt amplicon size 1203 bp
Locus specific primer 03wu: 5'-GATGGTTCATAGACCAGCAATACA-3'
2nd locus specific primer 16xd: 5'-CGTTACTTATCTGCACCACACAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11