Genotyping and primer details


Genotyping and primer details for 076B07 - At5g27530
Line ID 076B07
Locus At5g27530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9718523 of line 076B07 Chr5:9718523
   
Insertion allele amplicon 8474 - 07cp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07cp: 5'-AATTGTGCCTAAATCATTTGCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9717942
   
wt amplicon (over InsPos) 02dw - 07cp
wt amplicon start Chr5:9717942
wt amplicon end Chr5:9718804
wt amplicon size 863 bp
Locus specific primer 07cp: 5'-AATTGTGCCTAAATCATTTGCTTC-3'
2nd locus specific primer 02dw: 5'-AACTAATCCACGTTTGAGATTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11