Genotyping and primer details


Genotyping and primer details for 076F12 - At5g59260
Line ID 076F12
Locus At5g59260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23909335 of line 076F12 Chr5:23909335
   
Insertion allele amplicon 3144 - 57wz
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 57wz: 5'-CCAAAGTAATTGACCCACAAAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23910163
   
Insertion allele amplicon 8474 - KE19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE19: 5'-TCTCTGCCACATACTGTTTCATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23908728
   
wt amplicon (over InsPos) BH03 - KE19
wt amplicon start Chr5:23908728
wt amplicon end Chr5:23909762
wt amplicon size 1035 bp
Locus specific primer KE19: 5'-TCTCTGCCACATACTGTTTCATTC-3'
2nd locus specific primer BH03: 5'-TAACAAATAATACAAACACGGGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11