Genotyping and primer details


Genotyping and primer details for 078H09 - At4g32160
Line ID 078H09
Locus At4g32160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15532669 of line 078H09 Chr4:15532669
   
Insertion allele amplicon 8474 - 06sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06sf: 5'-TACTAGAGAACACAGTTGCGGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15532257
   
wt amplicon (over InsPos) 17tc - 06sf
wt amplicon start Chr4:15532257
wt amplicon end Chr4:15533182
wt amplicon size 926 bp
Locus specific primer 06sf: 5'-TACTAGAGAACACAGTTGCGGCTA-3'
2nd locus specific primer 17tc: 5'-ATTCCAACTTTGTTTGCAAGAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11