Genotyping and primer details


Genotyping and primer details for 081G07 - At1g70280
Line ID 081G07
Locus At1g70280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26466349 of line 081G07 Chr1:26466349
   
Insertion allele amplicon 8474 - 08cp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08cp: 5'-TCAGCCATGGTTGTATTCATCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26465686
   
wt amplicon (over InsPos) 09dy - 08cp
wt amplicon start Chr1:26465686
wt amplicon end Chr1:26466753
wt amplicon size 1068 bp
Locus specific primer 08cp: 5'-TCAGCCATGGTTGTATTCATCTTA-3'
2nd locus specific primer 09dy: 5'-AACATTCCAGACCATCTCTGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11