Genotyping and primer details


Genotyping and primer details for 082B07 - At2g19730
Line ID 082B07
Locus At2g19730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8512896 of line 082B07 Chr2:8512896
   
Insertion allele amplicon 8474 - 04dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04dr: 5'-AACAAGCCTAAGCTCTCTGTCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8512456
   
wt amplicon (over InsPos) 03ep - 04dr
wt amplicon start Chr2:8512456
wt amplicon end Chr2:8513407
wt amplicon size 952 bp
Locus specific primer 04dr: 5'-AACAAGCCTAAGCTCTCTGTCAAC-3'
2nd locus specific primer 03ep: 5'-AATTCTCAAGTTGGTTTTTGATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11