Genotyping and primer details


Genotyping and primer details for 082E05 - At2g28680
Line ID 082E05
Locus At2g28680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12303401 of line 082E05 Chr2:12303401
   
Insertion allele amplicon 3144 - 22by
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 22by: 5'-AATCTTCACCGGTTTCTCTACTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12303866
   
wt amplicon (over InsPos) 26da - 22by
wt amplicon start Chr2:12303004
wt amplicon end Chr2:12303866
wt amplicon size 863 bp
Locus specific primer 22by: 5'-AATCTTCACCGGTTTCTCTACTGA-3'
2nd locus specific primer 26da: 5'-AAGAAAACACCGGTCCATAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11