Genotyping and primer details


Genotyping and primer details for 083A06 - At4g26580
Line ID 083A06
Locus At4g26580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13412522 of line 083A06 Chr4:13412522
   
Insertion allele amplicon 8474 - KC39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KC39: 5'-CGAGGAAGCTACTAACGAGAGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13412067
   
wt amplicon (over InsPos) 11si - KC39
wt amplicon start Chr4:13412067
wt amplicon end Chr4:13413043
wt amplicon size 977 bp
Locus specific primer KC39: 5'-CGAGGAAGCTACTAACGAGAGGTA-3'
2nd locus specific primer 11si: 5'-AATGCCTCACTGATATATCCATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11