Genotyping and primer details


Genotyping and primer details for 083C06 - At5g15070
Line ID 083C06
Locus At5g15070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4879581 of line 083C06 Chr5:4879581
   
Insertion allele amplicon 8474 - 08sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08sj: 5'-ATTCTGTTTTAATCCATGACCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4879056
   
wt amplicon (over InsPos) 26tf - 08sj
wt amplicon start Chr5:4879056
wt amplicon end Chr5:4880065
wt amplicon size 1010 bp
Locus specific primer 08sj: 5'-ATTCTGTTTTAATCCATGACCAGG-3'
2nd locus specific primer 26tf: 5'-CCTCAGATCGTAGGAGATCAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11