Genotyping and primer details


Genotyping and primer details for 084E02 - At1g49450
Line ID 084E02
Locus At1g49450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18306885 of line 084E02 Chr1:18306885
   
Insertion allele amplicon 8474 - BH86
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH86: 5'-CACCAAATCGGTAAATCCTAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18306346
   
wt amplicon (over InsPos) 01dq - BH86
wt amplicon start Chr1:18306346
wt amplicon end Chr1:18307291
wt amplicon size 946 bp
Locus specific primer BH86: 5'-CACCAAATCGGTAAATCCTAGAAA-3'
2nd locus specific primer 01dq: 5'-CACACTTCCAAAGAAGATGACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11