Genotyping and primer details


Genotyping and primer details for 086F05 - At2g28350
Line ID 086F05
Locus At2g28350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12114871 of line 086F05 Chr2:12114871
   
Insertion allele amplicon 3144 - CB05
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CB05: 5'-TCCGCTACTGCTTCTACTCTAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12115157
   
wt amplicon (over InsPos) FI40 - CB05
wt amplicon start Chr2:12114319
wt amplicon end Chr2:12115157
wt amplicon size 838 bp
Locus specific primer CB05: 5'-TCCGCTACTGCTTCTACTCTAACC-3'
2nd locus specific primer FI40: 5'-AACTTCGTCTAGATGGAGCAAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11