Genotyping and primer details


Genotyping and primer details for 088C01 - At4g36940
Line ID 088C01
Locus At4g36940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17417963 of line 088C01 Chr4:17417963
   
Insertion allele amplicon 8474 - 02ed
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02ed: 5'-TTACTCAGAATCGAAGGTCCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17417475
   
wt amplicon (over InsPos) 14fi - 02ed
wt amplicon start Chr4:17417475
wt amplicon end Chr4:17418481
wt amplicon size 1007 bp
Locus specific primer 02ed: 5'-TTACTCAGAATCGAAGGTCCTGTT-3'
2nd locus specific primer 14fi: 5'-AAGTGTCTACAAGAGCGAGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11