Genotyping and primer details


Genotyping and primer details for 089F02 - At3g02570
Line ID 089F02
Locus At3g02570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:543539 of line 089F02 Chr3:543539
   
Insertion allele amplicon 8474 - 01ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01ht: 5'-TCAACTATGTGAAGCTCAATCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:544036
   
wt amplicon (over InsPos) 05io - 01ht
wt amplicon start Chr3:542948
wt amplicon end Chr3:544036
wt amplicon size 1089 bp
Locus specific primer 01ht: 5'-TCAACTATGTGAAGCTCAATCCTG-3'
2nd locus specific primer 05io: 5'-ACCAAGATCATGAAGGTAAAAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11