Genotyping and primer details


Genotyping and primer details for 090F10 - At1g52540
Line ID 090F10
Locus At1g52540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19571299 of line 090F10 Chr1:19571299
   
Insertion allele amplicon 8474 - 05hg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05hg: 5'-CAGATTCGATACATTCTGGTGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19570705
   
wt amplicon (over InsPos) 11nv - 05hg
wt amplicon start Chr1:19570705
wt amplicon end Chr1:19571741
wt amplicon size 1037 bp
Locus specific primer 05hg: 5'-CAGATTCGATACATTCTGGTGAGA-3'
2nd locus specific primer 11nv: 5'-CTTTGATCCGTTTTGCTATTCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11