Genotyping and primer details


Genotyping and primer details for 091B09 - At5g09410
Line ID 091B09
Locus At5g09410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2923138 of line 091B09 Chr5:2923138
   
Insertion allele amplicon 8474 - 06ke
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ke: 5'-GCTTGAACAGTGAGTCTGAGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2922694
   
wt amplicon (over InsPos) 02ks - 06ke
wt amplicon start Chr5:2922694
wt amplicon end Chr5:2923491
wt amplicon size 798 bp
Locus specific primer 06ke: 5'-GCTTGAACAGTGAGTCTGAGTTTG-3'
2nd locus specific primer 02ks: 5'-TTGAAAATTACACACAGAGCCAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11