Genotyping and primer details


Genotyping and primer details for 095B03 - At1g47980
Line ID 095B03
Locus At1g47980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17693317 of line 095B03 Chr1:17693317
   
Insertion allele amplicon 3144 - 74cs
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 74cs: 5'-GGAGTTTCCATTGAATTTGGAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17693678
   
wt amplicon (over InsPos) 10dy - 74cs
wt amplicon start Chr1:17692830
wt amplicon end Chr1:17693678
wt amplicon size 849 bp
Locus specific primer 74cs: 5'-GGAGTTTCCATTGAATTTGGAGTA-3'
2nd locus specific primer 10dy: 5'-GAGACCAACATAAGGGACCAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11