Genotyping and primer details


Genotyping and primer details for 097A03 - At1g10300
Line ID 097A03
Locus At1g10300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3378198 of line 097A03 Chr1:3378198
   
Insertion allele amplicon 8474 - BH75
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH75: 5'-AGGACATCCACAGATCAAGAGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3378759
   
wt amplicon (over InsPos) 25sq - BH75
wt amplicon start Chr1:3377969
wt amplicon end Chr1:3378759
wt amplicon size 791 bp
Locus specific primer BH75: 5'-AGGACATCCACAGATCAAGAGAGT-3'
2nd locus specific primer 25sq: 5'-TGTGTGGATCTCGAAGTTAGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11