Genotyping and primer details


Genotyping and primer details for 104B04 - At5g19410
Line ID 104B04
Locus At5g19410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6547409 of line 104B04 Chr5:6547409
   
wt amplicon (over InsPos) 10bs - KA29
wt amplicon start Chr5:6546423
wt amplicon end Chr5:6547456
wt amplicon size 1034 bp
Locus specific primer KA29: 5'-GAGTTTCGACTATCCAAACCAGAG-3'
2nd locus specific primer 10bs: 5'-TTAGCATGTTGATACCATTGGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11