Genotyping and primer details


Genotyping and primer details for 105E08 - At3g47800
Line ID 105E08
Locus At3g47800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17635581 of line 105E08 Chr3:17635581
   
Insertion allele amplicon 8474 - KT20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KT20: 5'-CTAGATTTACCTTCTTCACCGTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17635886
   
wt amplicon (over InsPos) 12sq - KT20
wt amplicon start Chr3:17635074
wt amplicon end Chr3:17635886
wt amplicon size 813 bp
Locus specific primer KT20: 5'-CTAGATTTACCTTCTTCACCGTCG-3'
2nd locus specific primer 12sq: 5'-AGCTCACAAGAGGAAGCTTAAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11