Genotyping and primer details


Genotyping and primer details for 106D02 - At3g60530
Line ID 106D02
Locus At3g60530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22373694 of line 106D02 Chr3:22373694
   
Insertion allele amplicon 3144 - 09ns
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 09ns: 5'-CACATTCTTTTCACCTCATTCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22372893
   
wt amplicon (over InsPos) 07or - 09ns
wt amplicon start Chr3:22372893
wt amplicon end Chr3:22373981
wt amplicon size 1089 bp
Locus specific primer 09ns: 5'-CACATTCTTTTCACCTCATTCTCA-3'
2nd locus specific primer 07or: 5'-CCACAAGCGTTACAAAGTGTTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11