Genotyping and primer details


Genotyping and primer details for 107A07 - At5g04740
Line ID 107A07
Locus At5g04740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1369208 of line 107A07 Chr5:1369208
   
Insertion allele amplicon 8474 - 03kq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03kq: 5'-TGTTTTAGAGCAAACAAAAGCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1368688
   
wt amplicon (over InsPos) 09li - 03kq
wt amplicon start Chr5:1368688
wt amplicon end Chr5:1369754
wt amplicon size 1067 bp
Locus specific primer 03kq: 5'-TGTTTTAGAGCAAACAAAAGCTCA-3'
2nd locus specific primer 09li: 5'-CCTGCATTGTCAGTTGTTACAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11