Genotyping and primer details


Genotyping and primer details for 109C05 - At2g33170
Line ID 109C05
Locus At2g33170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14056867 of line 109C05 Chr2:14056867
   
Insertion allele amplicon 8474 - KE10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE10: 5'-GATAGCTACATTGTCGGTAGAGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14057369
   
Insertion allele amplicon 3144 - KE11
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KE11: 5'-AACACTACTTCTCTCATCGTCGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14056456
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11