Genotyping and primer details


Genotyping and primer details for 109G08 - At2g06005
Line ID 109G08
Locus At2g06005 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:2332608 of line 109G08 Chr2:2332608
   
Insertion allele amplicon 8474 - 07dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07dl: 5'-AAACATACATGCACAGCTTCAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:2333155
   
wt amplicon (over InsPos) 19ec - 07dl
wt amplicon start Chr2:2332155
wt amplicon end Chr2:2333155
wt amplicon size 1001 bp
Locus specific primer 07dl: 5'-AAACATACATGCACAGCTTCAAAC-3'
2nd locus specific primer 19ec: 5'-TTCGGATAAATATCTGGATTTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11