Genotyping and primer details


Genotyping and primer details for 113G07 - At1g50260
Line ID 113G07
Locus At1g50260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18619834 of line 113G07 Chr1:18619834
   
Insertion allele amplicon 8474 - 27jn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27jn: 5'-CACAGAATACGTACTCAACGCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18618983
   
wt amplicon (over InsPos) 21vl - 27jn
wt amplicon start Chr1:18618983
wt amplicon end Chr1:18620074
wt amplicon size 1092 bp
Locus specific primer 27jn: 5'-CACAGAATACGTACTCAACGCCTA-3'
2nd locus specific primer 21vl: 5'-CAATGTAAGTTCTGTTGTGGCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11