Genotyping and primer details


Genotyping and primer details for 115A12 - At3g25580
Line ID 115A12
Locus At3g25580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9293945 of line 115A12 Chr3:9293945
   
Insertion allele amplicon 8474 - 03fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03fp: 5'-TGTATTGTCTGATTCTTCCCTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9293355
   
wt amplicon (over InsPos) 13gf - 03fp
wt amplicon start Chr3:9293355
wt amplicon end Chr3:9294435
wt amplicon size 1081 bp
Locus specific primer 03fp: 5'-TGTATTGTCTGATTCTTCCCTGTG-3'
2nd locus specific primer 13gf: 5'-AAGTGTTGTTCGATTGTTGACTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11