Genotyping and primer details


Genotyping and primer details for 115C04 - At5g18550
Line ID 115C04
Locus At5g18550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6162331 of line 115C04 Chr5:6162331
   
Insertion allele amplicon 8474 - IL13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IL13: 5'-TGTTCCTGAGTTCATGAAAGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6162848
   
Insertion allele amplicon 3144 - IL14
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IL14: 5'-CATATGTTCAAAGCCCTTATGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6161789
   
wt amplicon (over InsPos) IL13 - IL14
wt amplicon start Chr5:6161789
wt amplicon end Chr5:6162848
wt amplicon size 1060 bp
Locus specific primer IL14: 5'-CATATGTTCAAAGCCCTTATGGTT-3'
2nd locus specific primer IL13: 5'-TGTTCCTGAGTTCATGAAAGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11