Genotyping and primer details


Genotyping and primer details for 116H09 - At3g01420
Line ID 116H09
Locus At3g01420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:161577 of line 116H09 Chr3:161577
   
Insertion allele amplicon 8474 - 07vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07vf: 5'-GAGATAGCTAAACCGTCTTCGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:161296
   
wt amplicon (over InsPos) 32vu - 07vf
wt amplicon start Chr3:161296
wt amplicon end Chr3:162386
wt amplicon size 1091 bp
Locus specific primer 07vf: 5'-GAGATAGCTAAACCGTCTTCGTCA-3'
2nd locus specific primer 32vu: 5'-TGAAGTACGAAGACATCTTCACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11