Genotyping and primer details


Genotyping and primer details for 119E09 - At3g22270
Line ID 119E09
Locus At3g22270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7875323 of line 119E09 Chr3:7875323
   
Insertion allele amplicon 8474 - 03wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03wm: 5'-TGCTTAGTTTTCGATGTTGTGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7874726
   
wt amplicon (over InsPos) 85ww - 03wm
wt amplicon start Chr3:7874726
wt amplicon end Chr3:7875975
wt amplicon size 1250 bp
Locus specific primer 03wm: 5'-TGCTTAGTTTTCGATGTTGTGTTC-3'
2nd locus specific primer 85ww: 5'-CTGTACAAAGGTTTTGAATGAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11